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Devising our own method for slow release... (Read 11813 times)
comedydood
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Re: Devising our own method for slow release...
Reply #40 - 09/25/11 at 00:18:01
 
I can obtain Poloxamer 407 in powerded form, which I could then add BAC water to, and then add that to the Melanotan. I wonder if that would work?
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comedydood
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Re: Devising our own method for slow release...
Reply #41 - 09/30/11 at 23:12:32
 
Okay read this attachment. Apparently you can prepare controlled released gels using ONLY poloxamer 407 powder, dissolved in cold water, and then mixed with the drug (in this case, melanotan peptide). They made the gels with 20-30% concentrations of p407.
 
So, theoretically, all we need is sterile p407, and a way to mix it with cold BAC water and melanotan in a sterile way.
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comedydood
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Re: Devising our own method for slow release...
Reply #42 - 09/30/11 at 23:24:31
 
So taking this further... we would mix 30g of P407 into 100mL of BAC water to get a 30% solution w/v, and then add whatever amount of peptide you want to dissolve over a 24 hour period (2-4 grams maybe).
 
You would do this with cold water, which would make the P407 solution be a liquid still... then, once you inject the liquid, the temperate of your body will cause it to turn into a gel, which will breakdown over a 24 hour period, releasing the peptide slowly.
 
From what I can tell by my research, this should work and be pretty straightforward...  
 
I can get P407, but it mostly comes in containers of 100g... so I'd have to mix in 300 ml of BAC water, and then pull out 100ml of the solution, insert it into the melanotan peptide vial, agitate, and then withdraw half of that resulting mixture, and inject it subcutaneously.  
 
That would give me a 5g solution of melanotan in a 30% w/v P407 gel, which should dissolve over a 24 hour period at a fairly linear rate.
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darkhorse
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Re: Devising our own method for slow release...
Reply #43 - 10/01/11 at 09:29:44
 
Good luck comedydood!
 
How are you sure that the substance will be sterile? If you need another trial volunteer I might be willing, so look as the substances are definately sterile.
 
BW
 
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Re: Devising our own method for slow release...
Reply #44 - 10/01/11 at 15:33:01
 
Here is some indication of melanotan actually being used with the P407 gel, at least experimentally:
 
http://ukpmc.ac.uk/abstract/MED/8877878/reload=0;jsessionid=976EC1989A8A795DC006 B22DE00546D0
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sirler84
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Re: Devising our own method for slow release...
Reply #45 - 10/01/11 at 16:38:46
 
comedydood, i'm looking forward to hear some results with the poloxamer 407. This could be a breakthrough for all users in this forum.
 
Do you have a reputable supplier for poloxamer 407? In germany you can purchase Poloxamer 407 over the counter in a pharmacy without prescription.
 
 
I've attached the P407/Melanotan paper!
 
Before injecting the mixture, please contact a professional and ask him about possible risks!! Contact the manufacturer of P407 and ask him if its product has the grade for injection.  
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« Last Edit: 10/01/11 at 16:59:40 by sirler82 »  
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comedydood
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Re: Devising our own method for slow release...
Reply #46 - 10/03/11 at 17:31:08
 
sirler, can you give me more information on the p407 that is available over-the-counter in germany? What form does it come in? What grade is it?
 
I'm sourcing it from China, and though I can find 99% purity at medicine grade, I'm still leery about injecting it. My plan would be to mix everything, keep it cool using freezer packs, and place it in a UV sterilizing container for a day or so.
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sirler84
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Re: Devising our own method for slow release...
Reply #47 - 10/03/11 at 18:32:09
 
Quote from comedydood on 10/03/11 at 17:31:08:
sirler, can you give me more information on the p407 that is available over-the-counter in germany? What form does it come in? What grade is it?


 
The company's name is Fagron (www.fagron.de). The form of poloxamer 407 is solid. I would suggest to email them and ask about grade etc. I haven't read anything in their specification sheets.  
 
 
Quote from comedydood on 10/03/11 at 17:31:08:

I'm sourcing it from China, and though I can find 99% purity at medicine grade, I'm still leery about injecting it. My plan would be to mix everything, keep it cool using freezer packs, and place it in a UV sterilizing container for a day or so.

 
There should be some preservatives in ur mixture.
 
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Re: Devising our own method for slow release...
Reply #48 - 10/04/11 at 16:14:25
 
Quote from sirler84 on 10/03/11 at 18:32:09:
Quote from comedydood on 10/03/11 at 17:31:08:
sirler, can you give me more information on the p407 that is available over-the-counter in germany? What form does it come in? What grade is it?



The company's name is Fagron (www.fagron.de). The form of poloxamer 407 is solid. I would suggest to email them and ask about grade etc. I haven't read anything in their specification sheets.


Quote from comedydood on 10/03/11 at 17:31:08:

I'm sourcing it from China, and though I can find 99% purity at medicine grade, I'm still leery about injecting it. My plan would be to mix everything, keep it cool using freezer packs, and place it in a UV sterilizing container for a day or so.


There should be some preservatives in ur mixture.


 
SOmer preservative? Why? isnīt it suppused to be release in 24 hours?
Would you need a commercial email to do it more trusted?
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Re: Devising our own method for slow release...
Reply #49 - 10/04/11 at 22:47:19
 
I'm still missing the point of what you guys hope to achieve from this that's so much more beneficial than regular subcut injections. As a health professional myself even I don't think I'd be messing around with it.
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comedydood
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Re: Devising our own method for slow release...
Reply #50 - 02/03/12 at 16:22:51
 
Just wanted to confirm that I haven't forgotten about this thread. I have poloxamer 407 ordered and on the way...
 
And to answer your question about WHY, check this out:
 
Quote:
The ip administration of the poloxamer formulation to guinea pigs demonstrated a prolonged plasma level of the peptide which is most likely due to a slower diffusion of melanotan-1 from the gel formed in the intraperitoneal cavity. Thus the poloxamer gels have the potential to increase the therapeutic efficacy of peptides and other drugs by prolonging the ip rate of input into the systemic circulation.

 
This is to be expected... why else would a 16mg implant have more results than 150mg of injections? Slow release is key...
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comedydood
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Re: Devising our own method for slow release...
Reply #51 - 02/20/12 at 04:28:37
 
Okay, some exciting news... I've received the Poloxamer 407. I have enough to do some experimenting. I've already mixed a small amount into some BAC water. The key to making it dissolve is getting the water temperature low (but not freezing) and letting it sit for awhile. It is liquid when it's cold, but when I leave it out at room temperature, it turns into a clear gel.
 
My plan is to use a small glass tube that I purchased, and mix in 200ml of BAC water, and 60g of Poloxamer 407... that will give me a 30% concentration. I'll then cool the mixture and stir it, allowing it to dissolve as much as possible. After that I'll put the tube at room temperature, and seal it. Then I will use a UV germicidal lamp and set the tube under it for several hours (which should disinfect it thoroughly).
 
Finally, I will cool the gel again, turning it into a liquid, and inject 200ml into the Melanotan II vial. Then, I will extract 20ml and inject it into my butt cheek.  
 
That should give me 1mg of Melanotan II that will dissolve and release over an 8 hour period. Next time I'll step it up to 2mg per injection, but that will be the highest I'll go.
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darkhorse
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Re: Devising our own method for slow release...
Reply #52 - 02/20/12 at 10:35:39
 
Fantastic news!  
 
Good luck with the experiment and please let us know the outcome.
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Re: Devising our own method for slow release...
Reply #53 - 02/20/12 at 11:28:28
 
Really really excited about this post   Smiley Smiley Smiley
But, Why with melanotan II ? slow release sides are unknow with melanotan II ...
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comedydood
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Re: Devising our own method for slow release...
Reply #54 - 02/20/12 at 13:55:28
 
I wanted to experiment with melanotan II anyways, so I ordered it this time... After thinking about it, there shouldn't be any need to use more than 1mg per injection... if it works, then I'm definitely going to switch to melanotan-1.
 
I should know after a week whether slow release works or not... That's how long I plan on testing... just one week. I'm anticipating pretty substantial results.
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Re: Devising our own method for slow release...
Reply #55 - 02/21/12 at 00:05:58
 
if i understand it right, you will inject the melanotan II at low temperature to use it liquid instead that at medium temperature in gel form, if is that right, i can advance you that it hurts.... at least untill it will achieve the body temperature.
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comedydood
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Re: Devising our own method for slow release...
Reply #56 - 02/21/12 at 00:08:42
 
Hmm... I was wondering about that. I take it you know from personal experience?
 
I'm probably going to inject about 10-20ml of gel... so not that much volume.
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Re: Devising our own method for slow release...
Reply #57 - 02/21/12 at 10:31:57
 
Well it should itchy you for a while more than hurt.
Under my own experience, shots right before take it out from the refrigerator are a little bit itchy.
Nothing to be worried about, I guess that if your shots has a good liquid quantity, the itchy will last longer then.
 
another option is try to concentrate more the solution.
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Re: Devising our own method for slow release...
Reply #58 - 02/21/12 at 11:39:57
 
really interesting! keep us update and good luck Smiley
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Re: Devising our own method for slow release...
Reply #59 - 02/21/12 at 13:57:28
 
This is indeed very Interesting. Good luck.
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