Melanotan.org - Afamelanotide (SCENESSE®), Melanotan II and Bremelanotide Forums for Shared Discussions - Devising our own method for slow release...

Melanotan.org - Afamelanotide, Melanotan II and Bremelanotide Forums about tanning injections and tanning implants

See the Melanotan.org - afamelanotide introductory page.

Site Established: June 1999
Forum Established: January 2004

Greetings to you our Visitor. Please Sign In or Join.

05/25/13 at 02:38:06

Search the Melanotan.org forums:

Home

Help

Join

Big Change Coming to the Melanotan.org Forums (click here)

*PRE-MIXED MELANOTAN PEPTIDES MAKE PEOPLE SICK*
*DO NOT BUY MELANOTAN PEPTIDES ADULTERATED WITH A FILLER LIKE MANNITOL OR THAT ARE OUT OF DATE - DEMAND HIGH PURITY >99% AND FRESHNESS*

* Seen report(s) about the Melanotan peptides? Click here. *
*Please read - Governmental Health Agency Warnings*

*How is Melanotan.org Funded?*

Melanotan.org - Afamelanotide (SCENESSE®), Melanotan II and Bremelanotide Forums for Shared Discussions › Melanotan-1 › General Melanotan-1 Discussions
(Moderator Group: Board Mod)

‹ Previous topic | Next topic ›

Pages: 1 2 3 4 5

Send Topic

Print

Devising our own method for slow release... (Read 11915 times)

Jacques Global Moderator -Forum Log Keeper- fighting stupdity Posts: 1124	Re: Devising our own method for slow release... Reply #20 - 09/13/11 at 19:51:48 Why don't you continue discussing it here? I'm sure many are curious, me included.
Back to top	Dunning-Kruger effect: Unskilled and Unaware of It Suppliers 101: Everything you need to know. IP Logged

comedydood -Melanotant- Got Melanotan.org? Posts: 39	Re: Devising our own method for slow release... Reply #21 - 09/13/11 at 19:54:34 Here is another site that produces PLGA: http://www.purac.com/Purac-Biomaterials/Products/Polymers-for-drug-delivery/DL-l actide-Glycolide-copolymers.aspx They even had the option to request a sample...
Back to top	IP Logged

RIURAO

-Melanotanner-
Donor

Melanotan o no?

Posts: 362
Gender: male

male

Re: Devising our own method for slow release...
Reply #22 - 09/14/11 at 13:01:27

Quote from paradiso on 09/08/11 at 22:55:55:

I would strongly advise against trying this..

For non-professionals in a non-lab setting, there's simply too much guesswork and too much that could go wrong. I might have misinterpreted the implant talk as well, but even if your were able to produce one you cannot insert it.

As mentioned, sterilisation is a big issue and the fact that you think it's ok to "kill 95% of the bacteria at home in the microwave" is quite alarming.

Sorry to rain on your parade, but again I would not recommend this.

Well I do not afirm that Microwave is a safe way, i just request opinion to the forum about a safe way to perform the sterilisation.

I understand that an implant needs a good sterilisation as it needs to be inside the body for 16 days, but the Gel would only last 24-48 hours , so does it need the same sterilastion? when we perform the mix of water with Melanotan , it is not a sterile enviroment but it is enought for a shot wich will last in the body for 2-4 hours.

The idea is not to produce a real implant, but a gel that could produce a slow but continuos release of the product, not a 16 days implant but a 24-48 hours gel.
I am a little bit bussy at the moment but let me time and i will search about the PLGA

Sin limites.
Without limits.

IP Logged

comedydood -Melanotant- Got Melanotan.org? Posts: 39	Re: Devising our own method for slow release... Reply #23 - 09/16/11 at 00:49:03 Another option is Poloxamer gel, which this company manufactures (pure, research grade): http://maroonbiotech.com/products/products.html
Back to top	IP Logged

comedydood

-Melanotant-

Got Melanotan.org?

Posts: 39

Re: Devising our own method for slow release...
Reply #24 - 09/16/11 at 00:59:16

This is an incredibly informative article that discusses the types of slow-release strategies that are available:

http://www.globalresearchonline.net/journalcontents/volume3issue1/Article%20001. pdf

I highly recommend you read it. Here is a quote:

Quote:

Encapsulation-type depot preparations:
This depot preparation is prepared by encapsulating drug
solids within a permeation barrier or dispersing drug
particles in a diffusion matrix. The release of drug
molecule is controlled by the rate of permeation across the
permeation barrier and the rate of biodegradation of the
barrier macromolecules. Both permeation barrier and
diffusion matrix are fabricated from biodegradable or
bioabsorbable macromolecules, such as gelatin, dextran,
polylacticacid, lactide-glycolide copolymers,
phospholipids, and long-chain fatty acids and glycerides.
Typical examples are naltrexone pamoate-releasing
biodegradable microcapsule, liposomes, and
norethindrone-releasing biodegradable lactide-glycolide
copolymer beads.

Email

IP Logged

Socalguy

-Melanotanner-

Got Melanotan.org?

Posts: 479
Gender: male

male

Re: Devising our own method for slow release...
Reply #25 - 09/16/11 at 16:29:52

Well these are all very interesting, but I don't think any of us has the technology to produce
a sterile non-degraded melanotan-1 encapsulation and a sterile delivery system to implant it.

I only hope Clinivel would be bought out or partner with a large drug company that could speed
up the FDA approval process. Clinivel said it would be comercial by mid 2009!

IP Logged

comedydood

-Melanotant-

Got Melanotan.org?

Posts: 39

Re: Devising our own method for slow release...
Reply #26 - 09/22/11 at 07:17:23

I'm not ready to give up just yet... there has to be a way to accomplish a sustained release of Melanotan over a 12-24 hour period.

Recently, a member posted that he "delivered" melanotan via suppository... he didn't make a substance, he just shot the water up his butt. He reported that he got the effects as injecting... hot flashes, etc.

So, what if we formulated our own suppository using a slow-release base? A common one is bees wax. Here are some instructions: http://pharmlabs.unc.edu/labs/suppository/bases.htm

This would be much safer I'm assuming that trying to formulate something that you inject... The goal would be to put in a suppository that is slow-released over 10 hours or so, and do that for one week... that should be almost the equivalent of the implant.

I'm convinced that sustained, slow release is the way to achieve the best results possible with this peptide.

Email

IP Logged

sirler84

Ex Member

Re: Devising our own method for slow release...
Reply #27 - 09/22/11 at 11:54:57

Indeed, there are several vehicles that can slow down the release of drugs. However, most of them are in research status and not available on market
My suggestion is, to mix the peptide with a hydrogel used in wrinkle treatement as a filler (e.g. Aquamid®). This hydrogel consists of 2.5% polyacrylamide and 97.5 % water, so the peptid should dissolve easily. The idea is that the peptide could incorporate itself in the gel structure followed by a controlled release of the peptide in to the blood circulation.
In theory, aquamid remains in ur body for years. If or how this polymer releases the peptide is unclear.

A more simple method (but less efficient) to slow down the release of melanotan would be to adhere a cold-patch at the injection side. This idea is based on the principle that motion of molecules depend on temperature. The higher the temperature the higher the kinetic energy. ("Brownian motion")

« Last Edit: 09/22/11 at 17:32:43 by sirler82 »

IP Logged

comedydood -Melanotant- Got Melanotan.org? Posts: 39	Re: Devising our own method for slow release... Reply #28 - 09/23/11 at 07:19:45 What about Juvederm? This can be purchased pretty easily online... It is a cross-linked hyaluronic acid gel that apparently takes 6 months to dissolve... that's probably too long?
Back to top	IP Logged

RIURAO

-Melanotanner-
Donor

Melanotan o no?

Posts: 362
Gender: male

male

Re: Devising our own method for slow release...
Reply #29 - 09/23/11 at 10:21:26

Quote from sirler84 on 09/22/11 at 11:54:57:

Indeed, there are several vehicles that can slow down the release of drugs. However, most of them are in research status and not available on market
My suggestion is, to mix the peptide with a hydrogel used in wrinkle treatement as a filler (e.g. Aquamid®). This hydrogel consists of 2.5% polyacrylamide and 97.5 % water, so the peptid should dissolve easily. The idea is that the peptide could incorporate itself in the gel structure followed by a controlled release of the peptide in to the blood circulation.
In theory, aquamid remains in ur body for years. If or how this polymer releases the peptide is unclear.

A more simple method (but less efficient) to slow down the release of melanotan would be to adhere a cold-patch at the injection side. This idea is based on the principle that motion of molecules depend on temperature. The higher the temperature the higher the kinetic energy. ("Brownian motion")

Quote:

What about Juvederm? This can be purchased pretty easily online...

It is a cross-linked hyaluronic acid gel that apparently takes 6 months to dissolve... that's probably too long?

I have also thought about it but the release for both products is too long, I think we should never think in to use a release longer than 48 hours, due to the inconvenient of the incubation of bacterias due to a possible contamination

Sin limites.
Without limits.

IP Logged

sirler84

Ex Member

Re: Devising our own method for slow release...
Reply #30 - 09/23/11 at 12:37:45

Quote from RIURAO on 09/23/11 at 10:21:26:

I have also thought about it but the release for both products is too long, I think we should never think in to use a release longer than 48 hours, due to the inconvenient of the incubation of bacterias due to a possible contamination

The most important thing is, that the gel is bateria free. Fillers for wrinkles treatment are applicable because they are already produced in a GMP proved lab and stored in a syringe, ready for injection.
If u have access to fillers like hyaluronic acid it's definitely worth a try to mix melanotan with the filler. However it's hard to predict the release of melanotan

IP Logged

RIURAO

-Melanotanner-
Donor

Melanotan o no?

Posts: 362
Gender: male

male

Re: Devising our own method for slow release...
Reply #31 - 09/23/11 at 13:11:53

I could get it, but are you sure that during the mix with the Melanotan it will be 100% bacteria free ? and the other think that concerns me is that if the release is too long the peptide can be degraded with unknow results, it could give a cronic allergy symptoms untill the end of the release... 2 months ? we know that it should me stable for at least 13-16 days that is the self live of the implant , but what about a longer implant ?

Sin limites.
Without limits.

IP Logged

sirler84

Ex Member

Re: Devising our own method for slow release...
Reply #32 - 09/23/11 at 13:37:51

Quote from RIURAO on 09/23/11 at 13:11:53:

I could get it, but are you sure that during the mix with the Melanotan it will be 100% bacteria free ? and the other think that concerns me is that if the release is too long the peptide can be degraded with unknow results, it could give a cronic allergy symptoms untill the end of the release... 2 months ? we know that it should me stable for at least 13-16 days that is the self live of the implant , but what about a longer implant ?

If u have a preloaded syringe with filler, u just have to transfer the filler into ur melanotan vial. The possibility that it gets contaminated over this short distance is minimal. The peptide is degraded into single amino acids which then enter the amino acid metabolism in ur body.

There are insuline preparation which are zinc based or have protaminesulfate. They can release the insuline over 24 hours.

IP Logged

RIURAO

-Melanotanner-
Donor

Melanotan o no?

Posts: 362
Gender: male

male

Re: Devising our own method for slow release...
Reply #33 - 09/23/11 at 15:29:33

OK it seems that the contamination could be solved as you explain, but i have not very clear yet the theme about the degradation of the peptide, if the filler has a long self life how could be possible to guarantee the stability of the melanotan, as you know its stability at room temeprature is not very long... and if the release is longer than X days would it be safe?

Sin limites.
Without limits.

IP Logged

comedydood

-Melanotant-

Got Melanotan.org?

Posts: 39

Re: Devising our own method for slow release...
Reply #34 - 09/23/11 at 16:54:59

Yes, my main concern is the same thing as yours... even a 3-6 month degradation time is too long. I'd prefer something that was anywhere from 1 day to 1 month (on the high end).

I'm curious as to whether just injecting a small amount of the filler would decrease the degradation time to around a week? I know it also depends on where you inject it... sites with more blood flow will degrade it faster.

And finally, when using these dermal fillers, most doctors also carry the enzyme that breaks it down: hyaluronidase. They inject this and it dissolves the hydraulic acid within 4-7 days.

Quote:

Within 4 to 7 days of hyaluronidase injection, 80% of the NASHA gel [filler] had clinically disappeared at the hyaluronidase site

http://thebeautyrules.com/hyaluronidase-to-correct-dermal-filler-mistakes/

Unfortunately, I don't have a source for hyaluronidase just yet.

Email

IP Logged

sirler84

Ex Member

Re: Devising our own method for slow release...
Reply #35 - 09/23/11 at 19:12:16

Quote from comedydood on 09/23/11 at 16:54:59:

Yes, my main concern is the same thing as yours... even a 3-6 month degradation time is too long. I'd prefer something that was anywhere from 1 day to 1 month (on the high end).

I'm curious as to whether just injecting a small amount of the filler would decrease the degradation time to around a week? I know it also depends on where you inject it... sites with more blood flow will degrade it faster.

And finally, when using these dermal fillers, most doctors also carry the enzyme that breaks it down: hyaluronidase. They inject this and it dissolves the hydraulic acid within 4-7 days.

Quote:

Within 4 to 7 days of hyaluronidase injection, 80% of the NASHA gel [filler] had clinically disappeared at the hyaluronidase site

http://thebeautyrules.com/hyaluronidase-to-correct-dermal-filler-mistakes/

Unfortunately, I don't have a source for hyaluronidase just yet.

This could probably work but the main question remains unclear: Does melanotan incorporate itself into the gel matrix?

IP Logged

comedydood -Melanotant- Got Melanotan.org? Posts: 39	Re: Devising our own method for slow release... Reply #36 - 09/24/11 at 07:51:36 Well hyaluronic acid is hydrophilic, so I would imagine that the melanotan peptide would dissolve in the hyaluronic acid gel. Alternatively, you could put a small amount of BAC water in the melanotan vial, let it dissolve, then inject the gel into the vial as well.
Back to top	IP Logged

sirler84

Ex Member

Re: Devising our own method for slow release...
Reply #37 - 09/24/11 at 10:54:52

Quote from comedydood on 09/24/11 at 07:51:36:

Well hyaluronic acid is hydrophilic, so I would imagine that the melanotan peptide would dissolve in the hyaluronic acid gel. Alternatively, you could put a small amount of BAC water in the melanotan vial, let it dissolve, then inject the gel into the vial as well.

The peptide needs to be entrapped into the gel matrix physically!

IP Logged

comedydood

-Melanotant-

Got Melanotan.org?

Posts: 39

Re: Devising our own method for slow release...
Reply #38 - 09/24/11 at 17:34:17

This has been posted before, but what about getting a lab to formulate a slow release gel using P407?

http://www.google.com/url?sa=t&source=web&cd=2&ved=0CEUQFjAB&url =http%3A%2F%2Fmelanotan.org%2Fyabbfiles%2FAttachments%2FMT-_Controlled_release.pdf&rct=j&q=melanotan%20injection%20gel&ei=qAR-Tu7wCcLfsQL0lr0O&usg=AFQjCNFmVKxFvps50jXAgOEod5svG1ynCA&cad=rja

Email

IP Logged

sirler84

Ex Member

Re: Devising our own method for slow release...
Reply #39 - 09/24/11 at 18:53:17

Quote from comedydood on 09/24/11 at 17:34:17:

This has been posted before, but what about getting a lab to formulate a slow release gel using P407?

http://www.google.com/url?sa=t&source=web&cd=2&ved=0CEUQFjAB&url =http%3A%2F%2Fmelanotan.org%2Fyabbfiles%2FAttachments%2FMT-_Controlled_release.pdf&rct=j&q=melanotan%20injection%20gel&ei=qAR-Tu7wCcLfsQL0lr0O&usg=AFQjCNFmVKxFvps50jXAgOEod5svG1ynCA&cad=rja

It's pretty hard to find such a lab if u don't know somebody personally.

IP Logged

Pages: 1 2 3 4 5

Send Topic

Print

Melanotan.org - Afamelanotide (SCENESSE®), Melanotan II and Bremelanotide Forums for Shared Discussions › Melanotan-1 › General Melanotan-1 Discussions
(Moderator Group: Board Mod)

‹ Previous topic | Next topic ›

Melanotan.org - Afamelanotide (SCENESSE®), Melanotan II and Bremelanotide Forums for Shared Discussions » Powered by YaBB 2.1!
YaBB © 2000-2005. All Rights Reserved.

Valid XHTML

Valid CSS

Powered by Perl

Source Forge

"SCENESSE" is a registered brand trademark of Clinuvel Pharmaceuticals Ltd.
The Melanotan.org site and forums are not sponsored, endorsed or otherwise affiliated with Clinuvel Pharmaceuticals Ltd.