Quote from comedydood on 07/04/11 at 07:20:09:

 
B12 injections are actually given intramuscularly, I don't think injecting subcut over the buttocks will make any difference.

Well there you go, can be done both ways.
 
The ones I give are always intramuscular, as is the recommendation in Australia.

intersesting i might try this, it may give a better outcome because of a slower absorbion rate, although it sounds like a pain in the ass!  (sorry  )

RIURAO, Scenesse just consists of a biodegradable polymer PLGA (a mixture of lactic acid and glycolic acid) and melanotan-1. U can control the release (or half life of PLGA) by using different percentage of glycolic or lactic acid in PLGA. Clinuvel only mixes the peptide with the PLGA and presses it into a solid rice-grain like formulation.
 
If u mix PLGA with water it builds a gel. Then u would need to add the melanotan and then inject it. However, u need to be sure that no bacteria are present in ur mixture. So u need to incorporate an antibacterial agent.  
 

For me it is could be easy to get it , are you interested darkhorse ?
 
I probably will give it a try, any suggestion for the antibacterial agent ?  
Does it results in a very dense gel, or could it be injected  with with a insulin syringe?
I also guess that it has to be injected in fat parts ?
Could it be possible to manage the density of the finished product ?

Quote from Scott Stevenson on 09/02/11 at 12:13:44:

 
Yes, u are right, Scott. Clinuvel produces its implant in an aseptic facility to ensure a bacteria free formulation. However, if u want to make this implant at home i strongly advise to add some antibacterial agents. The most effective way would be to incorporate an broad spectrum antibiotic, e.g. norfloxacin etc into ur mixture.  
 
Clinuvels uses an extrusion machine, that presses the mixture through a small hole resulting in long sausage-like product. Finally, it is cut into smaller pieces that give a small implant.
 
Extrusion machines are often used in meat preparations. I guess, u can find similar machines in the housewares section in ur supermarket.  
 
 
 
Literature:
 
1.  D. Chitkara et al., Biodegradable injectable in situ depot-forming drug delivery systems,
     Macromol. Biosci. 6(2006). pp. 977-990.  
2. M. van de Weert et al., Semisolid, Self-catalyzed Poly(Ortho Ester)s as Controlled-Release  
    Systemes: Protein Release and Protein Stability Issues, J. Pharm. Sci. 91(2002b). pp. 1065-
    1074.  
3. A. Hatefi, B. Amsden, Biodegradable injectable in situ forming drug delivery systems, J.  
   Control. Release 80(2002). pp. 9-28.  
4. S.J. de Jong et al., Physically crosslinked dextran hydrogels by stereocomplex formation of  
   lactic acid oligomers: degradation and protein release behavior, J. Control. Release  
   71(2001). pp. 261-275.  
5. C.B. Packhaeuser et al., In situ forming parenteral drug delivery systems: an overview, Eur.  
    J. Pharm. Biopharm. 58(2004). pp. 445-455.  
6. M. Luck et al., Plasma protein adsorption on biodegradable microspheres consisting of  
    poly(D,L-lactide-co-glycolide), poly(L-lactide) or ABA triblock copolymers containing  
    poly(oxyethylene). Influence of production method and polymer composition, Journal of  
    Controlled Release. 55(2-3), (1998). pp. 107-20.  
7. K.J. Brodbeck et al., Phase inversion dynamics of PLGA solutions related to drug delivery  
   Part II. The role of solution thermodynamics and bath-side mass transfer, J. Control.  
   Release 62(1999). pp. 333-344.  
8. H. Kranz, R. Bodmeier, A novel in situ forming drug delivery system for controlled  
    parenteral drug delivery, Int. J. Pharm. 332(2007). pp. 107-114.  

I also understand that an anti bacterial component is needed for an implant wich will stay in your body at 36,5ºC during 14 days, it is perfect culture medium for any bacterial, but what if the gel or implant has a self life of let say 24-48 hours, probably an amount of 1 mg in daily injections ( Gel) could be enought and mimic the scen3sse implant as close as possible, we could have a constant release during 24 hours, aprox.

I can.

Maybe I'm misunderstanding here. This method would not result in an 'implant'. It would be an injectable gel. The method will involve mixing melanotan-1 with bacteriostatic water (as usual). Next adding bacteriostatic water to PLGA and then adding the two solutions together to 'thicken up' the mixture an a single, injectable gel.  
 
Important questions: How is the PLGA packaged? Is it in a sterile container/vial? Is it a dry powder? Would it mix with bac water to form a solution. And finallly, would said solution pull into a syringe when mixed with bacteriostatic water?
 
If the answer is yes to all of the above then I see no reason why this could not be acheived.

This company produces pharmaceutical grade PLGA: http://www.wako-chem.co.jp/specialty/plga/index.htm
 
I'm interested in trying this. RIURAO - where are you with this? Contact me via PM and we can talk about specifics.