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INOVEOL PHLO: new self-tanner acting on cAMP pathw (Read 11396 times)
sirler84
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #40 - 07/10/11 at 12:03:03
 
Yager, like beebeep already mentioned there are many enhancers for skin permeation.  
 
Joe, any updates?
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #41 - 07/10/11 at 14:08:54
 
there is another carrier, maybe as effective as "dmso", it is called: "emu oil".
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #42 - 07/10/11 at 14:30:04
 
Quote from yager on 07/10/11 at 14:08:54:
there is another carrier, maybe as effective as "dmso", it is called: "emu oil".

 
I mixed this: http://www.nutraplanet.com/product/nutraplanet/penetrate-8oz-8-oz.html   with 20% forskolin I ordered from smartpowders.com and got no results after using it for a long time.
 
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #43 - 07/10/11 at 14:51:23
 
"dmso" is often used as a topical.
 
typical forskolin preparations use dmso as a carrier.
 
such a solution could have a relatively low amount of "dmso", the rest could be any other enhancer of choice ie.  "emu oil, coconut oil".
 
some amount of dmso is required.
 
this study has delivered forskolin using such a solution:
 
mcb[dot]berkeley[dot]edu/labs/zucker/PDFs/Cheung_JNeurobiol566,273.pdf
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #44 - 07/10/11 at 14:57:28
 
here are the exact specifications for an effective forskolin solution:
 
mpbio[dot]com/detailed_info.php?family_key=02194803&country=223
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #45 - 07/10/11 at 21:42:39
 
Quote from yager on 07/10/11 at 14:57:28:
here are the exact specifications for an effective forskolin solution:

mpbio[dot]com/detailed_info.php?family_key=02194803&country=223

 
can't find it on the page, would you copy the ingredients and amounts?
It's also been used in a eyelash darkening serum named lipocils from talika
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #46 - 07/10/11 at 23:11:51
 
Quote:
Use one of the following procedures to make solutions:

Add anhydrous DMSO (10 ul) to 1 mg of the compound and vortex well. A clear homogeneous solution will be obtained. This will give a 10% (w/v), 213 mM solution in DMSO.
Add DMSO (128 ul) to 1 mg (2.134 umol) of the compound and vortex well to dissolve it completely. Then add water (2.006 ml) to the DMSO solution and vortex well. This will give a 1 mM solution in 6% DMSO (aqueous).


Stock solutions can be kept aliquoted and stored at -20oC or below for approximately 6 months.

Important Notes:

Always dissolve in DMSO first.
Always add water to the solution in DMSO. The reverse sequence (i.e. adding DMSO solution to water) may result in precipitation.
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yager
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #47 - 07/11/11 at 00:00:28
 
Solubility:
Forskolin: Soluble at 6 mg per ml in 95% ethanol and at 2.5 mg per 5 ul in anhydrous dimethylsulfoxide (DMSO). Various solvents, including ethanol, may inhibit the forskolin activation of adenylate cyclase. DMSO is the recommended solvent in preparing forskolin solutions because at concentrations of 5% or less of DMSO, there is little if any inhibition of forskolin activation.

Forskolin, 7-o-Hemisuccinyl-7-deacetyl: Use one of the following procedures to make solutions:
1. AddanhydrousDMSO(10ul)to1mgofthecompoundandvortexwell.Aclearhomogeneoussolutionw
illbeobtained.Thiswillgivea 10% (w/v), 213 mM solution in DMSO.
2. AddDMSO(128ul)to1mg(2.134umol)ofthecompoundandvortexwelltodissolveitcompletely.T
henaddwater(2.006ml)tothe DMSO solution and vortex well. This will give a 1 mM solution in 6% DMSO (aqueous).
 
the solution will only be <6% dmso. the rest water.
 
where can pure concentrated and high quality forskolin be obtained?
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sirler84
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #48 - 07/11/11 at 07:24:42
 
Pure forskolin is pretty expensive. U can buy it at sigma-aldrich. Another grades of forskolin can be purchased from www.alibaba.com.
 
What grad did they use in this paper?
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #49 - 07/11/11 at 10:43:53
 
pure forskolin is absolutely not affordable. The one I ordered ( and I already came across research that used the 20 pct version) is the one they're selling at www.smartpowders.com
Maybe it has to be purified. the powder looks very dark brown.
 
don't know which one they're using in the eyelash serum but the serum costs about 30 euros, so I can't imagine they have been using the expensive version in it unless very little is needed to be effective.
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sirler84
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #50 - 07/11/11 at 22:08:11
 
Beebeep, how long was ur forskolin trial until u've stopped?
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #51 - 07/11/11 at 22:40:08
 
about 6 - 7 weeks, absolutely no results in skin or hair, did it on my arm and shaved that spot regularly. I already sent you the formula I got from a bodybuilding site, but maybe someone could elaborate on it ( don't know if it's a good formula, the guy claimed it should work and knew about my experiment with penetrate 2.0)
 
Gel phase
water 5oz
polymer sp thickner(sodium polyacrylate) .4oz
 
Solvent phase
91% isopropyl alcohol 2oz
dmge .8oz
propylene glycol .8oz
d-limonene .8oz
Forskolin 10g
Vitamin D3 5g
 
emulsion phase
emulsifying wax .8oz
extra virgin organic cocounut oil .2oz
extra virgin olive oil .2oz
sweet almond oil .2oz
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #52 - 07/12/11 at 07:12:46
 
if you read the site, they are very specific about how to produce the forskolin solution with dmso, other substances will stop forskolin from being able to activate melanocytes, this is stated in the post above.
 
also 20% forskolin powder seems sufficient, people have had results:
 
http://www[dot]longecity[dot]org/forum/topic/43421-good-results-with-topical-forskolin-melanocytes/
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #53 - 07/12/11 at 08:19:50
 
how would it be applied all over the body, would it be sprayed on?
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #54 - 07/12/11 at 09:49:43
 
Quote from yager on 07/12/11 at 08:19:50:
how would it be applied all over the body, would it be sprayed on?

 
I know the longecity person and at first he thought he had results but it was only a color change due to irritation, so please ignore that pathway or you will only get dissappointed
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #55 - 07/12/11 at 14:41:46
 
this topic is very very hard... without expirements it wont work
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #56 - 07/12/11 at 14:51:01
 
Quote from latino69 on 07/12/11 at 14:41:46:
this topic is very very hard... without expirements it wont work

 
someone has already started experimenting and a few others including myself are about to start
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yager
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #57 - 07/12/11 at 15:48:37
 
if you dont want to waste time you have to realize it will almost certainly only work with dmso.
 
here is the only way which was proved to work in studies:
 
Solubility:
Forskolin: Soluble at 6 mg per ml in 95% ethanol and at 2.5 mg per 5 ul in anhydrous dimethylsulfoxide (DMSO). Various solvents, including ethanol, may inhibit the forskolin activation of adenylate cyclase. DMSO is the recommended solvent in preparing forskolin solutions because at concentrations of 5% or less of DMSO, there is little if any inhibition of forskolin activation.
Forskolin, 7-o-Hemisuccinyl-7-deacetyl: Use one of the following procedures to make solutions:
1. AddanhydrousDMSO(10ul)to1mgofthecompoundandvortexwell.Aclearhomogeneoussolutionw
illbeobtained.Thiswillgivea 10% (w/v), 213 mM solution in DMSO.
2. AddDMSO(128ul)to1mg(2.134umol)ofthecompoundandvortexwelltodissolveitcompletely.T
henaddwater(2.006ml)tothe DMSO solution and vortex well. This will give a 1 mM solution in 6% DMSO (aqueous).
Stock solutions can be kept aliquoted and stored at -20oC or below for approximately 6 months.
Important Notes:
Always dissolve in DMSO first. Always add water to the solution in DMSO. The reverse sequence (i.e. adding DMSO solution to water) may result in precipitation.
 
--------------------------------------------------------------------------------
------------------------------

it is easy:
 
mix dmso with forskolin as per above amounts
then add water as per above amounts
 
note: if following the method above dmso will be about 6% or 5% or less of the entire solution, probably not enough to irritate.
 
only thing is maybe 20% forskolin powder not concentrated enough
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #58 - 07/12/11 at 17:24:27
 
what about the garlick smell you can get from dmso? and how can you make the 20 pct more active? the 95 pct versions are very very expensive unless only very small amounts are needed.
What do you think of the solution I gave you a few posts earlier? ( gel phase, emulsion phase,....)
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Re: INOVEOL PHLO: new self-tanner acting on cAMP p
Reply #59 - 07/12/11 at 17:36:34
 
the garlick smell shouldnt be too much because dmso is a low percentage of the solution, and the smell can be covered up later if there is any faint smell.
 
it was already proven to work, as shown above, then after it is working, there is plenty of opportunity to experiment with different variations/additions etc., but the main thing for now is to get it working.
 
just use the method above with as high a potency forskolin that can be reasonably afforded.
 
with 20% forskolin powder, multiply the forskolin in the directions above by 5x, and then add that amount of 20% forskolin powder.
 
follow the directions exactly according to the way it was already done before.
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